crenata /em inoculation, alterations in the proteome corresponded to a general increase in the amounts of proteins belonging to the defense-related category, such as proteinase inhibitors, PRs, cell walls modifying, ROS detoxifying enzymes, and enzymes involved in the synthesis of secondary metabolites. areas A, B, C and D that are enlarged below. Spots showing changes between treatments are indicated: circles show new or missing spots, triangles and reversed triangles the spots with increased and decreased intensity, respectively. Proteins were resolved on first-dimension, pH 3C10 non-linear gradient, and second dimensions, SDS-PAGE on a 12% gel. Molecular mass and p em I /em were calculated using the PD-Quest software and standard molecular excess weight markers. 1471-2164-10-294-S3.jpeg (114K) GUID:?EBAA7248-45E9-4CB3-B308-3293C02ED387 Additional file 4 (R)-(+)-Citronellal Coomassie-stained 2-DE gels from root extracts of control, non-inoculated and inoculated SA 27774 plants. Representative 2-DE gel (up) has been divided into areas A, B, C and D that are enlarged below. Spots showing changes between treatments are indicated: circles indicate new or missing spots, triangles and reversed triangles the spots with increased and decreased intensity, respectively. Proteins were resolved on first-dimension, pH 3C10 non-linear gradient, and second dimensions, SDS-PAGE on a 12% gel. Molecular mass and p em I /em were calculated using the PD-Quest software and standard molecular excess weight markers. 1471-2164-10-294-S4.jpeg (116K) GUID:?24820D41-B117-4052-A6B0-ECE88963CB81 Additional file 5 Silver-stained 2-DE gels from root extracts of SA 4087 and SA 27774 plants. Representative 2-DE gel (up) has been divided into areas A, B, C and D that are enlarged below. Spots showing changes between both genotypes are indicated: circles indicate new or missing spots, triangles and reversed triangles the spots with increased and decreased intensity, respectively. Proteins were resolved on first-dimension, pH 3C10 non-linear gradient, and second dimensions, SDS-PAGE on a 12% gel. Molecular mass and p em I /em were calculated using the PD-Quest software and standard molecular excess weight markers. 1471-2164-10-294-S5.jpeg (126K) GUID:?5AA08CCE-6542-4728-9AA8-5DA6144C1A8A Additional file 6 Silver-stained 2-DE gels from root extracts of control, non-inoculated and Rabbit Polyclonal to PYK2 inoculated SA 4087 plants. Representative 2-DE gel (up) has been divided into areas A, B, C and D that are enlarged below. Spots showing changes between treatments are indicated: circles indicate new or missing spots, triangles and reversed triangles the spots with increased and decreased intensity, respectively. Proteins were resolved on first-dimension, pH 3C10 non-linear gradient, and second dimensions, SDS-PAGE on a 12% gel. Molecular mass and p (R)-(+)-Citronellal em I /em were calculated using the PD-Quest software and standard molecular excess weight markers. 1471-2164-10-294-S6.jpeg (123K) GUID:?4E0F7F23-F9AC-4072-927D-069F2072740E Additional file 7 Silver-stained 2-DE gels from root extracts of control, non-inoculated and inoculated SA 27774 plants. Representative 2-DE gel (up) has been divided into areas A, B, C and D that are enlarged below. Spots showing changes between treatments are indicated: circles indicate new or missing spots, triangles and reversed triangles the spots with increased and decreased intensity, respectively. Proteins were resolved on first-dimension, pH 3C10 non-linear gradient, and second dimensions, SDS-PAGE on a 12% gel. Molecular mass (R)-(+)-Citronellal and p em I /em were calculated using the PD-Quest software and standard molecular excess weight markers. 1471-2164-10-294-S7.jpeg (121K) GUID:?42C8C8A3-3CC0-4856-AD5E-99F4AD48D43E Additional file 8 Quantitative data for the spots detected in Coomassie stained gels showing differences between genotypes. 1471-2164-10-294-S8.doc (67K) GUID:?FD526470-B107-4420-A43A-442626F7C9A7 Additional file 9 Quantitative data for the spots detected in Coomassie stained gels showing differences between control and inoculated SA 4087 plants. 1471-2164-10-294-S9.doc (61K) GUID:?D707968E-5F3C-48AE-BD88-CF9B4F993796 Additional file 10 Quantitative data for the spots detected in Coomassie stained gels showing differences between control and inoculated SA 27774 plants. 1471-2164-10-294-S10.doc (50K) GUID:?589BBC04-96E9-4825-8BD2-55CB0CFE7547 Additional file 11 Quantitative data for the (R)-(+)-Citronellal spots detected in silver stained gels showing differences between genotypes. 1471-2164-10-294-S11.doc (104K) GUID:?563F696F-E8CC-47FC-9FAF-454AC4629272 Additional file 12 Quantitative data for the spots detected in silver stained gels showing differences between control and inoculated SA 4087 plants. 1471-2164-10-294-S12.doc (63K) GUID:?FC439AFA-0AB4-4ADD-B206-F03EC3CE8D51 Additional file.
