Next, to determine if MITF directly regulates PTEN expression through proximal enhancers we performed Cleavage Less than Targets and Launch Using Nuclease (CUT&RUN) with antibodies to MITF, as previously described,50 and to H3K27Ac, revealing active chromatin, and against H3K4me3, revealing active and poised promoters, in SK28 cell lines that were crazy type for (MITF-WT) or …
Continue reading Next, to determine if MITF directly regulates PTEN expression through proximal enhancers we performed Cleavage Less than Targets and Launch Using Nuclease (CUT&RUN) with antibodies to MITF, as previously described,50 and to H3K27Ac, revealing active chromatin, and against H3K4me3, revealing active and poised promoters, in SK28 cell lines that were crazy type for (MITF-WT) or had loss of function mutations in all alleles of it (MITF) (Fig