We prepared chromatin ingredients from mitosis-arrested HeLa cells using the FANCG-mutated forms and subjected these to SDS-PAGE

We prepared chromatin ingredients from mitosis-arrested HeLa cells using the FANCG-mutated forms and subjected these to SDS-PAGE. the phosphorylation sites of FANCG at mitosis also to assess their functional importance. Reasoning a potential kinase could be cdc2, that was reported to bind to FANCC previously, we showed that cdc2 phosphorylated a 14-kDa fragment from the …
Continue reading We prepared chromatin ingredients from mitosis-arrested HeLa cells using the FANCG-mutated forms and subjected these to SDS-PAGE