[23], studying the relationship between microorganisms and intestinal health, found a marked reduction of mucosa thickness induced from the proliferation ofC. and serum concentration of IgG, as well as improving the gut morphology without influencing the hematology guidelines in broiler rabbits. 1. Intro The constant antibiotic applications, as additives for animal feed, could provoke an increase of the number of resistant strains, as well as an enormous risk of crossed resistance spreading to additional microorganisms [1]. Although antibiotics have been used to a lesser degree in rabbits, compared with poultry and pigs, the use of antibiotics as zinc-bacitracin has been applied to reduce the proliferation ofClostridium perfringensand additional pathogens, mainly after weaning [2]. In the interests of public health, especially the prevention of the indiscriminate use of dietetic antibiotics, which have been the subject of worldwide issues, some antibiotic-alternatives that have had positive effects on growth performance and product quality should be used in the diet programs of nonruminant animals [3]. Natural products are considered better alternatives to Antibiotic Growth Promoters (AGP), from the point of look at GDC-0349 of biosafety and low residue [4]. TheAgavegenus, a part of the Agavaceae family, is native to Mexico. They have been cultivated since the pre-Columbian era for the production of textile materials, alcohol, molasses, pulp, and fodder, as well as for erosion control and dirt conservation [5]. Specifically, the stem of theAgave fourcroydeshas a high oligosaccharide content material, which forms a polydisperse combination [6]. Also, our earlier studies have shown the presence of flavonoids, anthocyanins, saponins, coumarins, reducing sugars, and tannins with this flower material [7]. On the other hand, the dried-stem powder ofAgave tequilanaA. tequilanaandA. fourcroydeswith the diet programs of mice decreased the cholesterol and glucose serum levels, as well as increasing the production of volatile fatty acids and the growth of lactic acid bacteria [6, 9]. There are several chemical benefits ofAgave A. fourcroydesin animal nutrition, especially on the diet of rabbits. It can be hypothesised from earlier studies that diet supplementation with this natural product may have the potential capability of stimulating the immune system and modulating the intestinal integrity, therefore improving the growth overall performance in rabbits. Thus, the objective of the current study was to evaluate the effects of dried-stem powder ofAgave fourcroydes A. fourcroydesAgave fourcroydeswas kindly offered for the study by the Study Center of Animal Production, Faculty of Veterinary Medicine, University or college of Granma, Cuba. Table 1 Compositions and nutrient levels in basal diet programs (as-fed basis). % mainly because fed10)/leukocytes.(iii) MCV: Ht (%) 10/RBCs (hundreds of thousands/mm3).The serum concentration of IgG was determined using a commercially available 125I Radio Immunoassay analyser kit having a. fourcroydes < 0.05) the final BW, ADG, and ADFI compared with BD (Table 2). However, F/G did not show significant variations (> 0.05) among treatments. Table 2 Effects of diet supplementation of powder on growth overall performance of broiler rabbits (95 days older). valuepowder= 16. (i) BW: body weight. (ii) ADFI: normal daily feed intake. (iii) ADG: normal daily gain. (iv) F/G: feed/gain percentage. SEM: standard error of the mean. 3.2. Gut Morphology Table 3 illustrates the data from the analysis of NEK5 the gut morphology of broiler rabbits at 95 days old. In the duodenum and cecum, theAgave fourcroydespowder improved (< 0.05) the muscle and mucosa thickness compared with BD, as well as improving the (< 0.05) GDC-0349 height and width of villi. However, the duodenum crypts depth ofA. fourcroydes< 0.05) than that of BD group (Table 3). In the mean time, the width and depth of cecum did not show significant variations (> 0.05) amongst the treatments. Table 3 Effects of diet supplementation of powder on gut morphology of broiler rabbits (95 days older). valuepowder= 8. SEM: standard error of the mean. 3.3. Hematology Guidelines and Serum Concentration of IgG Diet supplementation withA. fourcroydespowder did not influence (> 0.05) the hematology guidelines of broiler rabbits according to Table 4. It can also be seen that these guidelines GDC-0349 ofA. fourcroydes< 0.05) when rabbits were fed with theA. fourcroydespowder as feed additives (Number GDC-0349 1) compared with the BD group. Open in a separate window Number 1 Effect of diet supplementation of 1 1.5%Aoffered fourcroydespowder on concentration of IgG of broiler rabbits (95 days old) (SEM 0.319; value = 0.049). The experiment lasted for 60 days; = 8. Table 4 Effects of diet supplementation of powder on hematology guidelines of broiler rabbits (95 days old). value= 8. A. fourcroydespowder supplementation at 1.5% led to a higher.
